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alamarblue reagent #dal1100  (Thermo Fisher)


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    Thermo Fisher alamarblue reagent #dal1100
    Knockdown of MT1G , AKAP12 and MAFF in human primary Müller glia. A. MT1G , AKAP12 and MAFF siRNA knockdown in primary Müller glia exposed to light stress. B & C . <t>AlamarBlue</t> cell viability assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA treatment in response to light stress. n = 6 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. Data are presented as means ± standard error of the mean (SEM). D & E . JC1 assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA knockdown in response to light stress. n = 8 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. F – H . Volcano plots of differential gene expression (p < 0.05, FC > 1.5) in MT1G , AKAP12 and MAFF siRNA knockdown vs. control groups. I – K . IPA of differential gene expression in human primary Müller glia with MT1G , AKAP12 and MAFF siRNA knockdown compared to the control group in response to light stress.
    Alamarblue Reagent #Dal1100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alamarblue+reagent/alamarblue/pmc12178937-446-38-43
    Average 90 stars, based on 1 article reviews
    alamarblue reagent #dal1100 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability"

    Article Title: Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability

    Journal: Redox Biology

    doi: 10.1016/j.redox.2025.103691

    Knockdown of MT1G , AKAP12 and MAFF in human primary Müller glia. A. MT1G , AKAP12 and MAFF siRNA knockdown in primary Müller glia exposed to light stress. B & C . AlamarBlue cell viability assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA treatment in response to light stress. n = 6 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. Data are presented as means ± standard error of the mean (SEM). D & E . JC1 assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA knockdown in response to light stress. n = 8 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. F – H . Volcano plots of differential gene expression (p < 0.05, FC > 1.5) in MT1G , AKAP12 and MAFF siRNA knockdown vs. control groups. I – K . IPA of differential gene expression in human primary Müller glia with MT1G , AKAP12 and MAFF siRNA knockdown compared to the control group in response to light stress.
    Figure Legend Snippet: Knockdown of MT1G , AKAP12 and MAFF in human primary Müller glia. A. MT1G , AKAP12 and MAFF siRNA knockdown in primary Müller glia exposed to light stress. B & C . AlamarBlue cell viability assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA treatment in response to light stress. n = 6 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. Data are presented as means ± standard error of the mean (SEM). D & E . JC1 assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA knockdown in response to light stress. n = 8 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. F – H . Volcano plots of differential gene expression (p < 0.05, FC > 1.5) in MT1G , AKAP12 and MAFF siRNA knockdown vs. control groups. I – K . IPA of differential gene expression in human primary Müller glia with MT1G , AKAP12 and MAFF siRNA knockdown compared to the control group in response to light stress.

    Techniques Used: Knockdown, Viability Assay, Control, Gene Expression

    Related Articles

    Incubation:

    Article Title: Investigating the antimicrobial and anticancer potential of culturable fungal endophytes isolated from the stems of Kirkia acuminata Oliv
    Article Snippet: .. The plates were then incubated at 37 °C, 5% CO 2 and 95% humidity for 24 h. After incubation, a stock solution of the alamarBlue reagent (ThermoFischer Scientific, USA) diluted 1:1 with assay medium was added at 20 μL/well to achieve a final concentration of 10% alamarBlue reagent in the test plates. ..

    Article Title: PRPS activity tunes redox homeostasis in Myc-driven lymphoma
    Article Snippet: .. 20 μL AlamarBlue reagent (Thermo Fisher Scientific #DAL1100) was added to each well to achieve a final concentration of 10 % (v/v), prior to incubation for 4 h s at 37 °C in a 5 % CO 2 incubator. .. Post-incubation, AlamarBlue fluorescence was measured via the BMG Labtech CLARIOStar microplate reader, with ex/em of 560nm/590 nm, respectively.

    Article Title: Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability.
    Article Snippet: .. Human primary Müller glia (P3) were transfected with AKAP12, MT1G, MAFF and control siRNA in a 96-well plate for three days, then starved overnight and exposed to light stress for 4 h. Cells were treated with AlamarBlue reagent (1:10 dilution, #DAL1100, ThermoFisher Scientific) and incubated at 37 ◦C for 4 h using the AlamarBlue cell viability assay kit. .. The fluorescence was then read using the microplate reader (Fluostar Omega, BMG Labtech) with the 544 nm excitation wavelength and 590 nm emission wavelength.

    Article Title: Investigating the antimicrobial and anticancer potential of culturable fungal endophytes isolated from the stems of Kirkia acuminata Oliv.
    Article Snippet: .. The plates were then incubated at 37 °C, 5% CO2 and 95% humidity for 24 h. After incubation, a stock solution of the alamarBlue reagent (ThermoFischer Scientific, USA) diluted 1:1 with assay medium was added at 20 μL/well to achieve a final concentration of 10% alamarBlue reagent in the test plates. ..

    Concentration Assay:

    Article Title: Investigating the antimicrobial and anticancer potential of culturable fungal endophytes isolated from the stems of Kirkia acuminata Oliv
    Article Snippet: .. The plates were then incubated at 37 °C, 5% CO 2 and 95% humidity for 24 h. After incubation, a stock solution of the alamarBlue reagent (ThermoFischer Scientific, USA) diluted 1:1 with assay medium was added at 20 μL/well to achieve a final concentration of 10% alamarBlue reagent in the test plates. ..

    Article Title: PRPS activity tunes redox homeostasis in Myc-driven lymphoma
    Article Snippet: .. 20 μL AlamarBlue reagent (Thermo Fisher Scientific #DAL1100) was added to each well to achieve a final concentration of 10 % (v/v), prior to incubation for 4 h s at 37 °C in a 5 % CO 2 incubator. .. Post-incubation, AlamarBlue fluorescence was measured via the BMG Labtech CLARIOStar microplate reader, with ex/em of 560nm/590 nm, respectively.

    Article Title: Investigating the antimicrobial and anticancer potential of culturable fungal endophytes isolated from the stems of Kirkia acuminata Oliv.
    Article Snippet: .. The plates were then incubated at 37 °C, 5% CO2 and 95% humidity for 24 h. After incubation, a stock solution of the alamarBlue reagent (ThermoFischer Scientific, USA) diluted 1:1 with assay medium was added at 20 μL/well to achieve a final concentration of 10% alamarBlue reagent in the test plates. ..

    other:

    Article Title: PRPS activity tunes redox homeostasis in Myc-driven lymphoma
    Article Snippet: AlamarBlue Reagent , Thermo Fisher Scientific , Cat #DAL1100.

    Article Title: Effect of Acoustic Pressure on Temozolomide-Loaded Oleic Acid-Based Liposomes and Its Safety to Brain Tissue
    Article Snippet: AlamarBlue ® reagent was purchased from Thermo Fisher (Waltham, MA, USA).

    Transfection:

    Article Title: Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability.
    Article Snippet: .. Human primary Müller glia (P3) were transfected with AKAP12, MT1G, MAFF and control siRNA in a 96-well plate for three days, then starved overnight and exposed to light stress for 4 h. Cells were treated with AlamarBlue reagent (1:10 dilution, #DAL1100, ThermoFisher Scientific) and incubated at 37 ◦C for 4 h using the AlamarBlue cell viability assay kit. .. The fluorescence was then read using the microplate reader (Fluostar Omega, BMG Labtech) with the 544 nm excitation wavelength and 590 nm emission wavelength.

    Control:

    Article Title: Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability.
    Article Snippet: .. Human primary Müller glia (P3) were transfected with AKAP12, MT1G, MAFF and control siRNA in a 96-well plate for three days, then starved overnight and exposed to light stress for 4 h. Cells were treated with AlamarBlue reagent (1:10 dilution, #DAL1100, ThermoFisher Scientific) and incubated at 37 ◦C for 4 h using the AlamarBlue cell viability assay kit. .. The fluorescence was then read using the microplate reader (Fluostar Omega, BMG Labtech) with the 544 nm excitation wavelength and 590 nm emission wavelength.

    Viability Assay:

    Article Title: Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability.
    Article Snippet: .. Human primary Müller glia (P3) were transfected with AKAP12, MT1G, MAFF and control siRNA in a 96-well plate for three days, then starved overnight and exposed to light stress for 4 h. Cells were treated with AlamarBlue reagent (1:10 dilution, #DAL1100, ThermoFisher Scientific) and incubated at 37 ◦C for 4 h using the AlamarBlue cell viability assay kit. .. The fluorescence was then read using the microplate reader (Fluostar Omega, BMG Labtech) with the 544 nm excitation wavelength and 590 nm emission wavelength.



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    The effect of the drugs on cellular metabolism was assessed by treating RPL5-WT (blue) and RPL5-I60V (red) clones with various anticancer drugs, primarily targeting translational machinery. Metabolic activity was evaluated, after a 48-hour treatment period, by <t>AlamarBlue</t> ™ assay and data presented as fold change vs not treated sample. Graphs represent the average of two experiments run in duplicate. MNK1: MNK1 inhibitor; MTF: metformin; SIL: silvestrol; HHT: homoharringtonine; ANI: anisomycin; RV: resveratrol; HYG B: hygromycin B; ARA-C: cytarabine. Statistical significance was assessed by two-way ANOVA with Sidak’s multiple comparisons test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    Image Search Results


    Knockdown of MT1G , AKAP12 and MAFF in human primary Müller glia. A. MT1G , AKAP12 and MAFF siRNA knockdown in primary Müller glia exposed to light stress. B & C . AlamarBlue cell viability assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA treatment in response to light stress. n = 6 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. Data are presented as means ± standard error of the mean (SEM). D & E . JC1 assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA knockdown in response to light stress. n = 8 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. F – H . Volcano plots of differential gene expression (p < 0.05, FC > 1.5) in MT1G , AKAP12 and MAFF siRNA knockdown vs. control groups. I – K . IPA of differential gene expression in human primary Müller glia with MT1G , AKAP12 and MAFF siRNA knockdown compared to the control group in response to light stress.

    Journal: Redox Biology

    Article Title: Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability

    doi: 10.1016/j.redox.2025.103691

    Figure Lengend Snippet: Knockdown of MT1G , AKAP12 and MAFF in human primary Müller glia. A. MT1G , AKAP12 and MAFF siRNA knockdown in primary Müller glia exposed to light stress. B & C . AlamarBlue cell viability assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA treatment in response to light stress. n = 6 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. Data are presented as means ± standard error of the mean (SEM). D & E . JC1 assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA knockdown in response to light stress. n = 8 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. F – H . Volcano plots of differential gene expression (p < 0.05, FC > 1.5) in MT1G , AKAP12 and MAFF siRNA knockdown vs. control groups. I – K . IPA of differential gene expression in human primary Müller glia with MT1G , AKAP12 and MAFF siRNA knockdown compared to the control group in response to light stress.

    Article Snippet: Human primary Müller glia (P3) were transfected with AKAP12 , MT1G , MAFF and control siRNA in a 96-well plate for three days, then starved overnight and exposed to light stress for 4 h. Cells were treated with AlamarBlue reagent (1:10 dilution, #DAL1100, ThermoFisher Scientific) and incubated at 37 °C for 4 h using the AlamarBlue cell viability assay kit.

    Techniques: Knockdown, Viability Assay, Control, Gene Expression

    The effect of the drugs on cellular metabolism was assessed by treating RPL5-WT (blue) and RPL5-I60V (red) clones with various anticancer drugs, primarily targeting translational machinery. Metabolic activity was evaluated, after a 48-hour treatment period, by AlamarBlue ™ assay and data presented as fold change vs not treated sample. Graphs represent the average of two experiments run in duplicate. MNK1: MNK1 inhibitor; MTF: metformin; SIL: silvestrol; HHT: homoharringtonine; ANI: anisomycin; RV: resveratrol; HYG B: hygromycin B; ARA-C: cytarabine. Statistical significance was assessed by two-way ANOVA with Sidak’s multiple comparisons test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Journal: bioRxiv

    Article Title: Ribosomal protein L5 (RPL5/uL18) I60V mutation is associated to increased translation and modulates drug sensitivity in T-cell acute lymphoblastic leukemia cells

    doi: 10.1101/2025.07.16.665036

    Figure Lengend Snippet: The effect of the drugs on cellular metabolism was assessed by treating RPL5-WT (blue) and RPL5-I60V (red) clones with various anticancer drugs, primarily targeting translational machinery. Metabolic activity was evaluated, after a 48-hour treatment period, by AlamarBlue ™ assay and data presented as fold change vs not treated sample. Graphs represent the average of two experiments run in duplicate. MNK1: MNK1 inhibitor; MTF: metformin; SIL: silvestrol; HHT: homoharringtonine; ANI: anisomycin; RV: resveratrol; HYG B: hygromycin B; ARA-C: cytarabine. Statistical significance was assessed by two-way ANOVA with Sidak’s multiple comparisons test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Article Snippet: Alamarblue TM reagent (ThermoFisher Scientific) was added directly into the culture media at a final concentration of 10%, and the plate was incubated for 4 hours at 37 °C, 5% CO 2 , after which fluorescence was measured in a Spark plate reader (TECAN).

    Techniques: Clone Assay, Activity Assay, Alamar Blue Assay